Table 1.

Constitutive endogenous modulation of auxin levels does not affect cell death

	Relative free IAA level	Harpin	Pss61 0.1 OD	Pss61 0.05 OD	Pss61 0.01 OD
Vector control	1×	+	+	+	-
IaaM #1	~7×	+	+	+	+
IaaL #3	~0.1×	+	+	+	+

Transgenic plants used were Nicotiana tabacum cv. Samsun NN expressing (i) the tryptophan monooxygenase gene (iaaM) from Agrobacterium tumefaciens, (ii) the IAA-lysine synthetase (iaaL) gene from Pseudomonas syringae subsp. savastanoi that inactivates free auxin by conjugating it to lysine, from a constitutive 35S promoter, or (iii) the control vector integrated plants [24,25]. Relative IAA levels are as reported in the original publications. The leaves of these plants were infiltrated with harpin or Pseudomonas syringae pv. syringae (Pss61) at OD600 indicated. Development of cell death symptom was monitored at different time points and recorded as present (+) or no cell death (-) at 36 h post infiltration. There were no differences in the timing of appearance of cell death symptoms in all cases indicated with a +.