Figure 1.
A3H, the only Z2-type human APOBEC3 cytidine deaminase, is a potent inhibitor of LINE-1 retrotransposition. A) 293T cells were cotransfected with either pL1RP-EGFP or the negative control pL1RP(JM111)-EGFP in the presence of APOBEC3 expression vector. FACS was carried out to analyze for positive retrotransposition events by detecting eGFP expression in 293T cells at 5 days post-transfection. B) Cell lysates were harvested from the LINE-1 retrotransposition assay 3 days post-transfection and analyzed by SDS-PAGE, followed by immunoblotting with anti-V5 and antitubulin antibodies.