Figure 2.

A3H is an inhibitor for Alu retrotransposition. A) HeLa-HA cells were cotransfected with an Alu construct tagged with the retrotransposition indicator cassette, a LINE-1 construct that lacks ORF1 coding sequence (ORF2Δneo), and an APOBEC3 expression or control empty vector. At 3 days post-transfection, the cells were subjected to neomycin selection for 11–12 days to select for neomycin-resistant colonies that were indicative of positive retrotransposition events. B) Cell lysates were harvested and analyzed by SDS-PAGE, followed by immunoblotting with anti-HA antibodies.