Figure 1.

Altered epithelial fluid transport in hypoxia and transcriptional repression of CFTR. A) Basolateral to apical fluid movement across T84 monolayers was measured in hypoxic (Hx; black bars) vs. normoxic epithelia (Nx; open bars) in absence or presence of 50 μM forskolin. B) Confluent T84 cells were exposed to normoxia or 6 and 18 h of hypoxia, and relative expression of CFTR was quantified by microarray analysis. C) Confirmation of CFTR hypoxic repression by real-time PCR in Caco-2 and T84 cells as indicated. Results are pooled from 3–5 individual monolayers in each condition from 3 separate experiments and expressed as means ± se; *P ≤ 0.05 vs. respective normoxic condition, **P ≤ 0.05 vs. respective unstimulated control.