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. 2009 Jan;23(1):204–213. doi: 10.1096/fj.08-110221

Figure 4.

Figure 4.

HIF-1-mediated alterations of water and ion movement in vitro. A) Fluid movement was determined in T84 cells constitutively overexpressing HIF-1 (HIFΔODD). A subset of cells was stimulated with forskolin (Fsk; 50 μM), and monolayers were exposed to either normoxia (Nx; open bars) or hypoxia (Hx; solid bars). Total amount of fluid on the apical surface was determined by weight. B) Measurement of 1 μM forskolin-stimulated electrogenic Cl− secretion, as a function of Isc. Cl− secretory responses were compared in WT vs. HIF-1-overexpressing (HIFΔODD) T84 cells subjected to normoxia or 24 h hypoxia. Data are means ± se; *P ≤ 0.05 vs. WT normoxia; **P ≤ 0.05 vs. respective WT condition. C) Calorimetric assay of iodine efflux in normoxic T84 cells constitutively overexpressing HIF-1 (HIFΔODD; striped bars) compared to WT T84 cells subjected to normoxia (open bars) or 24 h hypoxia (solid bars). Data are expressed as coefficient of iodine secretion J ± sem (μeq/h/cm2); *P ≤ 0.05 vs. normoxia. All represented data are calculated from 3 separate experiments; n ≥ 3 monolayers/condition.