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. Author manuscript; available in PMC: 2009 Feb 22.
Published in final edited form as: Cell. 2008 Aug 22;134(4):587–598. doi: 10.1016/j.cell.2008.06.032

Figure 6. Trex1 can metabolize endogenous retroelement DNA.

Figure 6

A) Schematic of the marked IAP element used to measure IAP retrotransposition efficiency. The L1 element was designed with a similar selectable cassette.

B) HeLa cells were transfected with mutant or wild-type marked IAP, alone or together with the indicated amounts of Trex1 expression vector.

C) HeLa cells were transfected with the marked L1 element, alone or with the indicated amounts of Trex1 expression vector.

D) HeLa cells were transfected with pCDNA3 (which encodes plasmid-based neomycin resistance), alone or with the indicated concentrations of each Trex1 expression vector.

E) HEK 293T cells were transfected with the WT IAP, alone or with the indicated concentrations of HA-tagged Trex1. GAG expression and processing 48 hours post transfection was visualized by Western blot.

F) Relative IAP retrotransposition efficiency in the presence of the indicated Trex1 expression vectors. For each Trex1 construct, the left bar is 0 ng, the middle bar is 250 ng, and the right bar is 500 ng of expression vector. Data are mean and SEM of triplicate measurements and are representative of five independent experiments.

G) Relative L1 retrotransposition efficiency was quantitated in the presence of the indicated mutant forms of Trex1. Data are representative of three independent experiments.