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. 2009 Jan 19;4(1):e4230. doi: 10.1371/journal.pone.0004230

Figure 2. Lack of Bmi1 expression correlates with reduced tumor growth but not incidence in BXB11 mice.

Figure 2

A) Paraffin embedded lung sections from BXB11 and Bmi1−/−BXB11 lungs were stained for Bmi1 (green) and DAPI (blue). Dotted white lines delineate tumors. Scale bar = 30 µm. B) H&E staining of lung sections from BXB11 and Bmi1−/−BXB11 mice at the age of two weeks and three months demonstrates reduced tumor burdon in Bmi1−/−BXB11 lungs. Scale bar = 200 µm. C and D) Analysis of tumor incidence and growth within the lungs of BXB11 and Bmi1−/−BXB11 mice. Data presented as Box-and-Whiskers plots, for details of data presentation see figure legend of Figure 1 (n≥4 animals per genotype and age, for details see experimental materials). Note that tumor incidence is increased by a factor of two when compared to Figure 1A) because of the FVB/N genetic background introduced through mating with the Bmi1−/− mice. n.d. = not determined because tumors were confluent. p-values were calculated using Student's t-test only p-values indicating significance are shown.