Table 1. RT-PCR Primer and Probe Sequences.

Gene	Forward and reverse primers (5′→3′)	Primer Conc. (nM)	Probe (5′→3′)	Probe Conc. (nM)
ALP	CGGAACTCCTGACCCTTGAC TGTTCAGCTCGTACTGCATGTC	300	TCGAAGAGACCCAATAGGT	150
BMP-2	TGCCCCCCTACATGCTAGAC TCCAAAGATTCTTCATGGTGGAA	300	SYBR Green	150
BMP-4	ACCACGAAGAACATCTGGAGAAC GCTGAGGTTAAAGAGGAAACGAAA	300	SYBR Green	150
BMP-6	AACCAAACTTTTCTTATCAGCATTTATCAAGTC ATACTACACGGGTGTCCAACAAAA	300	ATCAGCACAGAGACTCT	150
OC	CTGGCCGCACTTTGCAT CTGCACCTTTGCTGGACTCT	300	CACCTGCCTGGCCAGC	150
Runx2	TGGACCTCGGGAACCCA GCGGTCAGAGAACAAACTAGGTT	300	AAGGCACAGACAGAAGC	150
18S RNA (22)	CGGCTACCACATCCAAGGAA GCTGGAATTACCGCGGCT	150	CACCAGACTTGCCCTC	100

The underlined base pairs indicate an exon-exon junction in the mRNA sequence