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. 2003 Dec;23(23):8651–8667. doi: 10.1128/MCB.23.23.8651-8667.2003

FIG. 1.

FIG. 1.

Induction of TR3 by serum and EGF. (A) H460 cells were serum starved for 16 h, replaced with medium containing 10% FBS or treated EGF (200 ng/ml) for 3 h, and then analyzed for TR3 expression by immunoblotting. α-Tubulin expression served as a control for similar loading of proteins in each lane. One of three similar experiments is shown. (B) Effect of EGF and serum on lung cancer cell proliferation. H460 cells were seeded (1,000 cells/well) in 96-well plates and maintained in serum-free medium for 24 h. The cells were then grown in serum-free medium or medium containing 10% FBS or treated with EGF (200 ng/ml) for 48 h. Viable cell numbers in quadruplicate were then determined by the MTS assay. Cells maintained in serum-free medium were set to 100%. The increase in cell numbers after EGF and FBS stimulation was normalized relative to cell numbers maintained in serum-free medium. The bars represent averages ± mean from two experiments.