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. 2009 Jan 19;206(1):25–34. doi: 10.1084/jem.20082013

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© 2009 Huntington et al.

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Figure 3. — Ectopic expression of Bcl-xL mildly augments human NK cell reconstitution in vivo. Human fetal liver HSCs were infected with retrovirus encoding hBcl-xL and GFP or GFP alone and used to generate HIS mice. (A) 8 wk after engraftment, thymus, spleen, and bone marrow cellularities were counted and CD45⁺ cells were enumerated based on surface expression as determined by flow cytometry. (B) GFP expression within hCD45⁺ thymocytes of HIS mice 8 wk after engraftment was detected by flow cytometry. (C) The percentages of GFP⁺ cells within the human graft were enumerated by flow cytometry. (D) NK cells were enumerated based on the data in A and surface expression hCD45 and NKp46 determined by flow cytometry. (E) NK cell subsets were analyzed by flow cytometry to detect surface expression of CD56 and CD16. FACS plots are pre-gated on hCD45⁺ cells and are representative of three to four mice in each group. Data in A and D are mean ± SEM of 3–6 mice in each group. P values are given when statistically significant.