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. 2003 Dec;23(23):8626–8636. doi: 10.1128/MCB.23.23.8626-8636.2003

FIG. 5.

FIG. 5.

CFP-M1-5 recovers to a lesser extent than CFP-H1b in heterochromatin. (A) Representative immunofluorescence of AO3_1 CHO cells cotransfected with CFP-H1b (green) and YFP-Lac (red) as determined by FRAP analysis. YFP-Lac is targeted to a heterochromatic array containing lac repeats. An image was taken before bleaching (Pre), immediately after bleaching (0 s), and then every 0.5 s until the level of recovery within the bleached region stabilized. Bleached regions contained the entire heterochromatic array, as shown in panel A. (B) Quantitative analysis comparing the relative recoveries of CFP-H1b and CFP-M1-5 in either heterochromatin (CFP-H1b : Lac and CFP-M1-5 : Lac, respectively) or the portion of the nucleus not containing the array (CFP-H1b : Nuc and CFP-M1-5 : Nuc) (see inset). Each data point represents the mean of the levels of recovery, measured at 0.5-s intervals, of at least 10 cells from one experiment. CFP-M1-5 recovered to a lesser extent than CFP-H1b in the heterochromatic array (Lac) but not in general nuclear regions (Nuc). Experiments were done as described above. Error bars did not significantly overlap between results for CFP-H1b : Lac and those for CFP-M1-5 : Lac.