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. 2009 Jan 19;206(1):15–23. doi: 10.1084/jem.20081880

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© 2009 Serhan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — MΦs generate novel products. (A) Mouse-resident MΦs (5 × 10⁶ cells/ml) incubated with DHA or 14S-HpDHA showing targeted LC/MS/MS-based mediator lipidomics. Selected ion chromatogram (m/z 359/250) of 7,14-diHDHA (II) and its transconjugated isomer (I) is shown. Selected ion chromatogram (dashed overlay; m/z 359/250) shows double dioxygenation product 7S,14S-diHDHA. (inset) FACS plot of isolated resident MΦs. (B and C) Lipid mediator lipidomics. (B) Mass spectra for 7,14-diHDHA (m/z 359; B) and corresponding isomer (C). See inset and Results for diagnostic ions (n = 3). amu, atomic mass unit.