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. 2009 Jan 19;206(1):169–181. doi: 10.1084/jem.20081747

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© 2009 Schuster et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Not all HLA-DR⁺ epidermal leukocytes express CD1c at 9 wk EGA. (A) Flow cytometric analysis revealed the presence of CD45⁺CD1c⁺ cells in embryonic skin. Histograms display HLA-DR staining (black line) of CD45⁺CD1c⁺ (R1) and CD45⁺CD1c⁻ (R2). Gray line, isotype control. Shown are representative dot plots of three to five experiments per group. (B) Immunofluorescence triple labeling identified HLA-DR⁺CD1c⁻ (arrows) and HLA-DR⁺CD1c⁺ (not depicted) leukocytes in embryonic epidermis. Arrowheads denote CD45⁺HLA-DR⁺CD1c⁺ cells. Data are representative of at least three experiments per group. Bars, 50 μm.