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. 2009 Jan 19;206(1):169–181. doi: 10.1084/jem.20081747

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© 2009 Schuster et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Epidermal leukocytes sequentially acquire CD1c and Langerin. CD1c and Langerin expression in developing human skin was assessed by flow cytometry (A) and in situ by immunofluorescence staining (B). (A) Dot plots show the development of CD45⁺ cells with regard to the expression of CD1c and Langerin (2,500 CD45⁺ cells displayed) and are representative of three to five experiments per group. (B) Immunofluorescence triple labeling identifies CD45⁺CD1c⁺Langerin⁺ cells (middle, arrows) at 11 wk EGA. Arrowheads denote a CD45⁺CD1c⁻Langerin⁻ LC precursor. Data are representative of at least three experiments per group. Bars, 50 μm.