FIG. 7.

Examination of SWI/SNF complex integrity in swi/snf mutants by coimmunoprecipitation analysis. (A) SNF2-myc strains containing WT SWI/SNF subunits or the indicated swi/snf deletions were grown in YPD, and WCEs were prepared as described in Materials and Methods. Aliqouts containing 0.3 to 0.5 mg of protein were immunoprecipitated with mouse monoclonal myc antibodies. Ten percent of the input samples (Input), 100% of the immunoprecipitates (Ppt), and 10% of the supernatant (Sup) fractions were resolved by 4 to 20% SDS-PAGE and subjected to Western blot analysis using monoclonal mouse antibodies against myc or rabbit antibodies against Snf5p. The upper and lower portions of each membrane were probed separately with different antibodies. (B) The same kind of analysis as that described for panel A was carried out by using the WT or snf2Δ strains harboring SWI3-myc.