Figure 5.

LS skin fibroblasts from psoriatic lesions release bioactive chemerin. (A) Blood-purified pDC were preincubated with 300 pM of recombinant human chemerin at 37°C. Cells were then washed and tested in Transwell assay for their ability to transmigrate an endothelial cell monolayer in response to medium (Ctrl), 100 pM chemerin, or LS supernatant (1:81 dilution). Data were obtained with supernatants obtained from five different donors. *, P < 0.05. Horizontal lines indicate mean values for each experimental group. (B) H460 cells transfected with the empty vector (mock) or ChemR23 were stimulated with fibroblast-derived supernatant from LS skin (1:81 dilution) or healthy donors (HS; 1:9 dilution). Anti-ChemR23 mAb was used at 10 μg/ml. Western blots were performed as detailed in Materials and methods.