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. 2009 Jan 19;206(1):153–167. doi: 10.1084/jem.20081202

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© 2009 Li and Eckhardt

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Spleen and BM B cell profiles in homozygous V_HEμ^a and V_HΔ^a mice. (A) Flow cytometry profiles of splenic lymphocytes from adult mice stained for B220 (B cell marker) and CD3-ε (T cell marker). (B) Surface IgM^a and relative Igμ transcript levels in splenic B cells. Left, histogram of spleen cells stained with antibody to IgM^a. Right, quantitative RT-PCR results, using primers for B1-8i μ mRNA and normalized to hgprt1 mRNA (see Materials and methods). μ mRNA from V_HEμ^a B cells are set as 1.0 and WT^b/WT^b is the negative control. (C) BM B cell subsets. Top, BM cells gated for B220⁺ cells and analyzed for CD43 and IgM expression. Bottom, BM B220⁺ cells analyzed for IgD and IgM. Data shown are representative of three animals of each genotype.