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. 2009 Jan 19;206(1):153–167. doi: 10.1084/jem.20081202

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© 2009 Li and Eckhardt

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — IgM allotype expression in V_HEμ^a/WT^b and V_HΔ^a/WT^b mice. (A) Flow cytometry profiles of lymphocytes in BM, spleen, and the peritoneal cavity, stained with antibodies to IgM^a and IgM^b. (B) IgM allotype expression on immature and mature B cells of BM (enriched for B220⁺ cells). Plots are of cells gated for IgM^a alone (left), cells that were IgM^a+ and IgD⁻ (middle; immature B cells), or IgM^a+ and IgD⁺ cells (right; mature B cells) and then analyzed for IgM^a and IgM^b. In both A and B, the number in top right quadrant = (percentage of double-positive cells)/(total IgM⁺ cells). (C) Percentage of IgM^a+b+/IgM⁺ cells at different stages of development and in different tissues. n = number of mice analyzed. Error bars show SD.