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. 2009 Jan 19;206(1):43–49. doi: 10.1084/jem.20081438

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© 2009 Veldhoen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Independent RORγt and AhR regulation in Th17 cells. (A) FACS-sorted naive CD4 T cells retrovirally transduced with RV-GFP control, AhR-GFP, or RORγt-GFP vector as indicated on abscissa were cultured in the presence (shaded bars) or absence (open bars) of FICZ. As a comparison, FACS-sorted naive CD4 T cells cultured under Th17 condition in the presence (shaded bars) or absence (open bars) of FICZ are shown. The figure shows mRNA expression for the indicated genes normalized for HPRT mRNA expression. The figure is representative of three independent experiments. (B) FACS-sorted naive CD4 T cells were retrovirally transduced with either control vector containing truncated human CD4 (RV-hCD4Δcyto) together with RV-GFP control vector (top) or with Ahr-hCD4Δcyto together with RORγt-GFP (bottom) and cultured in the presence of FICZ. Expression of hCD4 versus GFP is shown on the left. Intracellular IL-22 expression in gated populations is shown on the right. Results represent three independent experiments.