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. 2009 Jan 6;106(2):492–497. doi: 10.1073/pnas.0807614106

Fig. 3.

Fig. 3.

Rapid turnover of single EB1 and CLIP-170 molecules on growing ends of microtubules. (A) Averaged intensity profiles of EB1–Alexa 488 and CLIP-170(H2)–GFP microtubule plus-end decoration (n = 20 for both). The concentrations of EB1–Alexa 488 and CLIP-170(H2)–GFP were 250 nM and 25 nM, respectively. (B–D) Histograms of dwell times of single binding events of 10 nM EB1–Alexa 488 (B), 5 nM CLIP-170(H2)–GFP in the absence of EB1 (C), or 5 nM CLIP-170(H2)–GFP in the presence of 250 nM unlabeled EB1 (D). Exponential fits to the data yielded mean lifetimes of interactions of 0.81 ± 0.06 s for EB1–Alexa 488 (n = 184), 2.69 ± 0.16 s for CLIP-170(H2)–GFP alone (n = 800), and 1.03 ± 0.03 s for CLIP-170(H2)–GFP in combination with EB1 (n = 753).