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. 2009 Jan 7;106(2):641–646. doi: 10.1073/pnas.0805165106

Fig. 5.

Fig. 5.

Wnt7a regulates CNS endothelial cell migration and expression of the BBB-specific transporter glut-1 (slc2a1). (A) Measurement of mouse brain endothelial cell (bEND3.0) migration across a fibronectin coated filter to a basal media, basal media containing VEGF, basal media containing Wnt, or basal media containing both VEGF and Wnt. Wnt7a is a potent migration factor for CNS endothelial cells. Error bars represent standard error of the mean. *<0.001 as analyzed by a two tailed standard T-test not assuming normal variance. (B) Table of GeneChip values, given in arbitrary units, for primary cultures of mouse brain endothelial cells grown in basal medium, or basal medium with Wnt7a. Wnt7a up-regulates expression of transcripts encoding molecular transporters (slc2a1, slc7a1, and slc7a5) but not tight junction molecules (Occludin, ZO-1) or pan-endothelial cell adhesion molecules (PECAM, VE-Cad). (C-F) Cross sections of the developing CNS of E11.5 endothelial-specific β-catenin mutants (E and F) and litter-mate controls (C and D) were stained with vascular marker BSL I (i, green) and an antibody directed against the BBB-specific glucose transporter glut-1 (ii, red). Glut-1 specifically stains vascular endothelial cells in the control animals, whereas glut-1 does not stain the vascular endothelial cells in the endothelial-specific β-catenin mutants but instead stains CNS parenchymal cells (iii, merged images). (Scale bar,100 μm.)