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. 2008 Sep 26;85(1):34–43. doi: 10.1189/jlb.0208101

Fig. 2.

Fig. 2.

Antigen presentation assay. (A) MACS-purified CD8+ T cells from 7-day-inoculated, 32-week ethanol-consuming or control BALB/c female mice were cultured with the LLO91–99 peptide-loaded P815 cell at a 5:1 ratio in the presence of Brefeldin A for 6 h. As a control, CD8+ T cells were cultured with P815 cells not loaded with peptide. Flow cytometry data of one water- and ethanol-administered group in the absence or presence of the LLO91–99 peptide. (B) CD8+ T cells from 7-day-inoculated, 28-week ethanol-consuming and matched control BALB/c female mice were cultured with LLO91–99 peptide-loaded P815 cells at different ratios in the presence of Brefeldin A for 6 h at 37°C. Responding CD8+ T cells were analyzed by intracellular IFN-γ staining. Control wells contained no P815 cells. (C) Statistical analysis of the data shown in A from all mice in the groups; N(H20) = 9; N(EtOH) = 6; ***, P < 0.001.