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. 2008 Sep 26;85(1):34–43. doi: 10.1189/jlb.0208101

Fig. 3.

Fig. 3.

Antigen-specific CD8+ T cell responses. (A) Representative flow cytometry plots of noninoculated and water- and ethanol-administered CD8+ T cells with or without the LLO91–99 peptide at 7 days post-LM ΔactA LACK inoculation. Flow cytometry plots are gated on CD8+ T cells. (B–D) BALB/c female mice consuming ethanol and water for 4–44 weeks were inoculated with LM ΔactA or LM ΔactA LACK; spleens were harvested at different days postinoculation and splenocytes cultured 6 h. Time course of LLO91–99-specific CD8+ T cells as percent responding (B, upper) and total number per spleen (B, lower) in water- and chronic ethanol-consuming mice as determined by intracellular IFN-γ staining. Four days, N(H2O) = 4; N(EtOH) = 6. Five days, N(H2O) = 3; N(EtOH) = 4. Seven days, N(H20) = 7; N(EtOH) = 6. Twelve days, N(H2O) = 8; N(EtOH) = 8. Twenty-nine days, N(H2O) = 8; N(EtOH) = 7. (C) p60217–225-specific and (D) p60449–457-specific CD8+ T cell responses at 7 days post-LM ΔactA LACK inoculation; N(H2O) = 7; N(EtOH) = 6; *, P < 0.05.