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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: J Leukoc Biol. 2008 Feb 19;83(5):1207–1217. doi: 10.1189/jlb.0607391

Fig. 5.

Fig. 5

MyD88 KO is required for neutrophil recruitment into the airways following S. rectivirgula exposure. WT and MyD88 KO (n=4 mice/group) were exposed to saline or S. rectivirgula (60 μg) once/day for 2 days and analyzed for neutrophil recruitment 24 h after the last exposure. (A) One lung lobe from individual mice was homogenized, and MPO activity was measured as described in Materials and Methods. (B) MIP-2 was measured in BALF recovered from individual mice by ELISA. (C) RT-PCR was performed on RNA isolated from one lung lobe from individual WT or MyD88 KO mice exposed to saline or S. rectivirgula using primers specific for TNF-α and IL-1β. The housekeeping gene β-actin was used as an internal control. *, P < 0.05, MyD88 KO exposed to S. rectivirgula compared with WT mice exposed to S. rectivirgula.