FIG. 1.

Longer telomeres elevate replicative capacity in TER1 K. lactis strains. (A) Genomic DNA was prepared from the indicated strains expressing TER1 from a low-copy-number plasmid (lane 1) or from a high-copy-number plasmid (lane 2). The blot was hybridized with a K. lactis-specific telomeric probe. (B) Percentage of surviving clones with relation to the number of passages in the strain carrying the low- or high-copy-number plasmid. (C) Genomic DNA was prepared from the indicated strains reaching their final cell doublings. Lanes 2 to 4, representative subclones produced from the strain that carried TER1 on a low-copy-number plasmid; lanes 5 to 7, representative subclones produced from the strain that carried TER1 on a high-copy-number plasmid; lane 1, genomic DNA prepared from the strain expressing TER1 from a low-copy-number plasmid prior to loss of the telomerase-bearing plasmid. The blot was hybridized with a K. lactis-specific telomeric probe.