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Rap1p binding is not essential for determination of replicative capacity in K. lactis. (A) Genomic DNA was prepared from strains expressing TER1 from a high-copy-number plasmid (WT), ter1-AA (AA), ter1-Bsi (Bsi), or ter1-Acc (Acc). The blot was hybridized with a K. lactis-specific telomeric probe. (B) Percentage of surviving clones with relation to the number of passages in the TER1, ter1-AA, ter1-Bsi, or ter1-Acc strain.
