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Rap1p binding is not important for determination of replicative capacity in S. cerevisiae. (A) Genomic DNA was prepared from S. cerevisiae strains carrying plasmids expressing TLC11 (WT) or tlc1-C476G (476G). The blot was hybridized with an S. cerevisiae-specific telomeric probe. (B) Percentage of surviving clones with relation to the number of passages in TLC11 or tlc1-C476G.
