TABLE 4.
Replicative capacity of telomerase-deficient K. lactis strains with reduced Rap1p binding potential is not affected by truncation of the C-terminal tail of Rap1p
| Strain | Initial telomere length (kb) | Range of final telomere length (kb) | Δ shortening (bp) | Replicative capacity (no. of passages)
|
|
|---|---|---|---|---|---|
| Avgc | Maximal | ||||
| rap1-ΔC TER1 | 0.675 | 0.19-0.21 | 505 | 4.79 ± 0.68 | 9 |
| rap1-ΔC ter1-AA | 6-10+a | 0.25-10+a | 5.75-9.75b | 21.6 ± 4.2 | 40 |
| rap1-ΔC ter1-Bsi | 6-10+a | 0.25-10+a | 5.75-9.75b | 18 ± 2 | 40 |
| rap1-ΔC ter1-Acc | 6-10+a | 0.25-10+a | 5.75-9.75b | 17.9 ± 0.37 | 39 |
a
The upper limit of the telomeric bands is at the limit of mobility of the gels, around 10 kb.
b
It is impossible to define the source of the fastest migrating fragments; therefore, the Δ shortening is shown for either possibility (fastest migrating fragment or slowest migrating initial fragment shortening to the fastest migrating fragments).
c
Data are means ± standard deviations.