TABLE 5.
Rap1p binding potential is not critical for determination of the replicative capacity of telomerase-deficient S. cerevisiae strains
| Strain | Initial telomere length (kb) | Range of final telomere length (kb) | Δ shortening (bp) | Replicative capacity (no. of passages)
|
|
|---|---|---|---|---|---|
| Avgd | Maximal | ||||
| TLC1 | 0.54 | 0.21-0.24 | 295 | 1.91 ± 0.31 | 2 |
| tlc1-C476G | 6-10+a | 6-10+a | NDb | 23.3 ± 3.8c | 80+ |
a
The upper limit of the telomeric bands is at the limit of mobility of the gels, around 10 kb.
b
Shortening cannot be seen at the gels' resolution.
c
Calculated only for those clones that achieved final replicative capacity.
d
Data are means ± standard deviations.