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. 2003 Dec;23(23):8416–8428. doi: 10.1128/MCB.23.23.8416-8428.2003

FIG. 1.

FIG. 1.

Nuclear localization of Lsh. (A) Lsh is localized in nuclear extracts. For analysis of Lsh expression the pre-B-cell line PD31, fetal thymus and embryonal fibroblasts (MEFs) were examined by Western analysis and probed with a specific antibody raised against the C-terminal portion of Lsh. Lanes: C, cytoplasmic extract; N, nuclear extract (equal loading of protein extracts). (B) Lsh is localized in overexpressed nuclear extracts. Lsh was induced in 3T3 cells in the GeneSwitch system and examined by Western analysis. Lanes: C, cytoplasmic extract; N, nuclear extract (equal loading of protein extracts). (C) Lsh is preferentially expressed during S phase. The T-cell line EL4 was synchronized with mimosine (M), aphidicolin (A), or nocodazole (N) or left untreated (Ctrl) and then examined by Western analysis for the presence of Lsh or β-actin. Lanes: C, cytoplasmic extract; N, nuclear extract (extracts were prepared from equal number of cells). The lower panel shows the result of the cell cycle analysis and the distribution of cells at different stages of the cell cycle.