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Nuclear localization domain of Lsh. (A) Construction map of Lsh deletional mutants. (B) Expression of deletion mutants. Nuclear extracts were examined 24 h after transfection by Western analysis with specific antiserum raised against the GFP peptide (equal loading of protein extracts). (C) The nuclear localization domain is N terminal. At 24 h after transfection with indicated plasmids, 3T3 cells were examined under a fluorescence microscope.
