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. 2003 Dec;23(23):8416–8428. doi: 10.1128/MCB.23.23.8416-8428.2003

FIG. 4.

FIG. 4.

Lsh localizes to pericentromeric heterochromatin. (A) Lsh predominantly associates with chromatin. 3T3 cells were extracted with Triton X, and different fractions examined by Western analysis with the indicated antibodies: lane 1, flushed fraction; lane 2, Triton X-resistant fraction after DNase digestion; lane 3, ammonium sulfate wash; lane 4, 2 M NaCl wash; lane 5, solubilized pellet. (B) Lsh colocalizes with DAPI. At 24 h after transfection, GFP-tagged Lsh was examined by fluorescence microscopy. (C) Lsh colocalizes with HP1α. At 24 h after transfection, GFP-tagged Lsh was immunostained for detection of HP1α.