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Lsh requires higher-order heterochromatin structure for localization. (A) Lsh dissociates from DAPI after TSA treatment and reassociates after recovery. After TSA treatment of 3T3 cells, Lsh was induced and cells were immunostained with anti-Flag antibody. (B) Dissociation of branched anti-H3-K9me from DAPI after TSA treatment and reassociation after recovery. The cells were also immunostained with the antibody raised against the branched H3-K9 methylated peptide.
