TABLE 1.
HP1 recovery after TSA treatmenta
| Time (h) | % HP1 recovery
|
|
|---|---|---|
| LSH+/+ cells | LSH−/− cells | |
| 0 | 25.2 | 33.0 |
| 3 | 58.5 | 62.5 |
| 6 | 71.9 | 74.5 |
| 12 | 78.9 | 86.9 |
a
MEFs derived from Lsh+/+ or Lsh−/− embryos were treated for 5 days with TSA (75 ng/ml), washed, and cultured for the indicated time periods. Cells were then stained with HP1α antibody and DAPI in order to monitor the recovery of higher-order chromatin structure in the absence of Lsh. The percentage indicates the percentage of cells in which HP1 colocalized with DAPI.