FIG. 8.
Perturbation of ribosomal biogenesis by actinomycin D (ActD) increases L11-HDM2-p53 complex levels. (A and B) WI38 cells were treated with 5 nM actinomycin D for 24 h (+) or left untreated (−) before being lysed for analysis for the expression of various proteins and protein complex formation. Approximately 500 ug of total protein was utilized for each IP. (C) WI38 cells were treated with the proteasome inhibitor MG132 for 6 h before lysis to stabilize HDM2 in the presence (+) or absence (−) of 5 nM actinomycin D. A total of 200 ug of protein was used in each IP. (D) WI38 cells were treated with UV (50 J/m2) (+) at 24 h before harvesting or left untreated (−), and IP was performed as described above.