TABLE 3.
Overexpression of IME2 partially suppresses the meiotic defect in cells lacking Cak1pa
| Strain | % Meiosis
|
|
|---|---|---|
| YEp351 | pS324 | |
| CDC28-43244 | 78 ± 12 | 88 ± 1 |
| cak1-Δ CDC28-43244 | 12 ± 8 | 47 ± 10 |
| GAL1-SIC1 CDC28-43244 | 57 ± 6 | 59 ± 3 |
| GAL1-SIC1 cak1-Δ CDC28-43244 | 35 ± 3 | 46 ± 7 |
a
The indicated homozygous diploid strains harboring a high-copy-number vector containing IME2 (pS324) or a control vector lacking IME2 (Yep351) were sporulated for 24 h before analysis. The completion of both meiotic divisions was determined as described in Materials and Methods.