TABLE 5.
Meiotic phenotypes of IME2 T-loop mutantsa
| Strain | % Meiosis | Spore formation |
|---|---|---|
| IME2 CDC28-43244 | 85 ± 1 | + |
| ime2-T242A CDC28-43244 | 86 ± 3 | + |
| ime2-Y244F CDC28-43244 | 53 ± 6 | − |
| ime2-T242A, Y244F CDC28-43244 | 17 ± 1 | − |
a
The wild-type IME2 chromosomal locus was replaced with mutant ime2 alleles, and the indicated homozygous diploid strains were generated. Cells were assayed after 48 h in sporulation medium. The completion of both meiotic division and the formation of spores were scored as described in Materials and Methods.