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. 2003 Dec;23(23):8440–8449. doi: 10.1128/MCB.23.23.8440-8449.2003

FIG. 2.

FIG. 2.

Mutations in the IFD region impair telomere maintenance. (A) Telomere lengths were determined for strains bearing wild-type or mutated TERT after the strains had been restreaked on the plate once. The identities of the mutants are indicated at the top of the panel. The location of Y′-type telomeres is indicated by brackets. The amplified Y′ fragments that are often observed in type I survivors are marked by a solid triangle. (B) Telomere lengths were determined for strains bearing wild-type EST2 (C874), the LYID589 mutant, or an empty vector after the strains were passaged in liquid culture by serial dilution (1:500 for each dilution). The amplified Y′ fragments that are often observed in type I survivors are marked by solid triangles. The heterogeneous telomere fragments observed in type II survivors are marked by vertical brackets. Samples that exhibit features of survivors are marked with + at the bottom. (C) Telomere lengths were determined for W303a strains with plasmids that overexpressed individual IFD mutant proteins. DNAs were isolated from clones that had been restreaked twice on plates after initial transformation. A strain that does not contain any overexpression plasmid (Control) was tested in parallel.