TABLE 2.
Junctional sequences observed in survivors of SLY19 and in the rad52 and yku70 gene deletion derivativesa
| Strain | Junctional sequence | Modifications | No. of overlapping bases | No. of events | |
|---|---|---|---|---|---|
| Parental | TTTATAAAATTATACTGTT | GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA | |||
| AAATATTTTAATATG | TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT | ||||
| SLY19 | TTTATAAAATTATA(CTGTT) | AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAGG | −5/0 | 1 | 1 |
| TTTATAAAATTATAC(TGTT) | (A) ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −4/−1 | 2 | 4 | |
| TTTATAAAATTATACTGT(T) | (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −1/−4 | 2 | 1 | |
| TTTATAAAATTATACTG(TT) | (AACAGTATAATTTTATAAACC) CTGGTTTTGGTTTTGTAGAG | −2/−21 | 3 | 1 | |
| TTTATAAAATTATA(CTGTT) | (AACAG) TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −5/−5 | 4 | 2 | |
| TTTATAAAATTATA(CTGTT) | (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG | −5/−12 | 10 (12) | 2 | |
| TTTATAAAATTATACTG(TT) | (AACAGTATAA---GAGTGGTTGACGAATAATTATG) CTGAAG | −2/−60 | 9 (10) | 29 | |
| TTTATAAAATTAT(ACTGTT) | (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG | −6/−53 | 9 (10) | 5 | |
| TTTATAAAAT(TATACTGTT) | (AACAGTATAA--------GTCTATGTATTTG) TATAAAATAT | −9/−293 | 8 | 1 | |
| EMK11 (yku70Δ) | TTTATAAAATTATACTG(TT) | (AACAGTATAA---GAGTGGTTGACGAATAATTATG) CTGAAG | −2/−60 | 9 (10) | 29 |
| TTTATAAAATTAT(ACTGTT) | (AACAGTATAA---GAGTGGTTGACGAAT) AATTATGCTGAAG | −6/−53 | 9 (10) | 1 | |
| EMK13 (rad52Δ) | TTTATAAAATTATACTGT(T) | (AACA) GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA | −1/−4 | 2 | 1 |
| TTTATAAAATTATA(CTGTT) | (AACAG) TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −5/−5 | 4 | 3 | |
| TTTATAAAATTATA(CTGTT) | (AACAGTATAATT) TTATAAACCCTGGTTTTGGTTTTGTAGAG | −5/−12 | 10 (12) | 4 | |
| TTTATAA(AATTATACTGTT) | (AACAG) TATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −12/−5 | 10 (12) | 2 | |
| GTTTATAAA(ATTATACTGTT) | (AACAGTATAA) TTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −11/−11 | 10 (12) | 8 | |
| AAAACCAGGGTTT(AT--TGTT) | (AACAGTATAATTTTATAAACCCT) GGTTTTGGTTTTGTAGAG | −16/−23 | 10 (12) | 2 | |
Shown are the nucleotide sequences at the sites of joining between two HO breaks in Ura− survivors of SLY19 and their mutant derivatives. The original sequences that constitute noncomplementary single-stranded ends are underlined. Microhomologies with overlapping sequences are shown in boldface. The deletions are shown in parentheses. Negative numbers in the “Modifications” column indicate the number of nucleotides deleted in either side of the DSBs. In cases of imperfect overlap, mismatches with several matching bases on either side were only considered as microhomologies. The number of overlapping bases is shown to indicate the total number of overlapping sequences (in parentheses) and the identical sequences among them.