TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| B. pseudomallei | ||
| PP844 | Prototroph, blood culture isolate from a patient at Khon kaen University Hospital | 37 |
| KN100 | PP844/pKBS1 | This study |
| PP844(pBBS1) | PP844 containing pBBS1 | This study |
| KN100(pBBS1) | PP844/pKBS1 containing pBBS1 | This study |
| Z2BS1 | PP844/pZ2BS1 containing single copy of rpoS-lacZ transcriptional fusion | This study |
| Z3BS1 | PP844/pZ3BS1 containing single copy of rpoS-lacZ translational fusion | This study |
| S. enterica serovar Typhimurium | ||
| BSST100 | Clinical isolate from a patient at Royal Hallamshire Hospital | This study |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK− mK+) supE44 λ−thi-1 relA1 gyrA96 | 13 |
| CC118λpir | Δ(ara, leu)7697 araD139 ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB (Rfr) argE(Am) recA1 λpir+ | 17 |
| JM83 | F−ara Δ(lac-proAB) rpsL(Smr) φ80dlacZΔM15 | 39 |
| SM10λpir | RP4-2-tet::Mu-1 (Kmr) thi-1 thr leu tonA lacY supE recA | 32 |
| S17-1λpir | RP4-2-tet::Mu-1 kan::Tn7 (Tpr Smr) thi proA hsdR recA | 32 |
| Y. enterocolitica | ||
| 08081c | Inv+ | 29 |
| Plasmids | ||
| pUCBS1 | pUC19 containing a 600-bp internal segment of B. pseudomallei rpoS | This study |
| pUCBS2 | pUC19 containing the full-length rpoS | This study |
| pKNOCK-Tc | Mobilizable suicide vector for construction of gene knockouts in Gram-negative bacteria | 2 |
| pKBS1 | pKNOCK-Tc containing a 600-bp internal segment of B. pseudomallei rpoS | This study |
| pBBR1MCS | Broad-host-range cloning vector, Cmr | 23 |
| pBBS1 | pBBR1MCS containing the full-length B. pseudomallei rpoS gene | This study |
| pZINT2 | Mobilizable integrative vector for construction of single-copy lacZ transcriptional fusions | 27 |
| pZINT3 | Mobilizable integrative vector for construction of single-copy lacZ translational fusions | This study |
| pZ2BS1 | pZINT2 containing the 5′ region of nlpD and 3′ region of rpoS fused to lacZ | This study |
| pZ3BS1 | pZINT3 containing the 5′ region of nlpD and 3′ region of rpoS fused to lacZ | This study |
| pFR97 | Plasmid vector for construction of lacZ translational fusions | 31 |