TABLE 2.

PCR primers

Primer	Sequence	Restriction sitea
SBKF	5′-GACGACTTCCGGGCGCTTCT-3′
SBKR	5′-ATCGTCGGGCAGCAGATCGAG-3′
RPOSF	5′-CCTGTCGATCCGCTGAAGTATT-3′
RPOSR	5′-CCAGAATCGGTGTCATTGATGAA-3′
PNSF	5′-GCCCGCATGCCGCTTTATCGGATCGCGCTCG-3′	SphI
PNSR	5′-GCCGTCTAGAAGAAGCGCCCGGAAGTCGTC-3′	XbaI
PNSFR97	5′-GCCGTCTAGAAAGCGCCCGGAAGTCGTCCAG-3′	XbaI

^aUnderlined in the sequence.