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. 2003 Dec;185(23):7019–7023. doi: 10.1128/JB.185.23.7019-7023.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 1. — Western blots of sodium dodecyl sulfate-10% polyacrylamide gels show the absence of IIA^Crr and EI in BAP2 and BAP3, respectively. Blotting was performed as described with polyclonal antibodies raised against His-tagged EI (Eurogentec) and His-tagged IIA^Crr (7). Ten micrograms of cellular protein prepared from cells grown on tryptic soy broth without dextrose or 10 ng of purified His-tagged protein was subjected to gel electrophoresis. (A) IIA^Crr-immunoreactive signal in cell extracts of M145 (lane 1), BAP2 (lane 2), BAP3 (lane 3), and His-tagged IIA^Crr (lane 4). (B) EI-immunoreactive signal in cell extracts of M145 (lane 1), BAP2 (lane 2), BAP3 (lane 3), and His-tagged EI (lane 4).