Figure 1.

(A) Image of the predicted structure of the switch tag peptide (ST) in its helical conformation. (B) Far UV CD spectra of ST. A 0.2 mg/ml solution of peptide in 50 mM NaH₂PO₄ buffer, pH 7.0 was used throughout. Increasing the quantity of the hydrophobic solvent TFE promoted the transition of peptide secondary structure from random coil to α-helix.

Abbreviation: TFE, tri-fluoro ethanol.