FIG. 1.

(A) Restriction enzyme map and genetic organization of the pvs and pvu loci of V. parahaemolyticus WP1, including the cognate vibrioferrin receptor gene (pvuA) and a receptor gene (psuA) for a siderophore of unknown origin (18). The transcriptional direction for each ORF is shown by an open arrow. D, F, and T indicate positions of disruption, putative Fur boxes, and termination signals, respectively. (B) Inserts of the relevant recombinant plasmids. pVP3151 was initially isolated as a clone conferring a Lac⁺ phenotype in the FURTA (53). The smaller solid bars with restriction enzyme names represent the insert fragment ligated into the suicide vector pKTN701 to construct the gene disruptants. (C) DIG-labeled probes constructed for hybridization experiments.