TABLE 2.

Primers and conditions used to confirm in-frame deletions

Deletion	Primers	Conditions
sopB	5′-CGGGATCCGCGTTACGCAATCACTATC	33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 5 min
	5′-GCGGCCGCCCGTTGACATCCTCCAGAA
sopE	5′-CGGGATCCTCTTGGCGCGTAGTCCTTC	33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 4.5 min
	5′-TTTGCGGCCGCGCACTGGATACGCTGAACGA
sopE2	5′-CGGGATCCGCGCAGGCGTTTAGAAGACAGTT	33 cycles of 94°C for 30 s, 54°C for 30 s, and 72°C for 5 min
	5′-GCGGCCGCGTTCCAGCATCAGCCACTTG
sipA	5′-GCGGCCGCACCTGGGGTTGAGTCCTAC	33 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 6 min
	5′-CCCGGGACACCAAGGCACGAG
invB	5′-GGGCGCCAAGAGAAAAAGATGTC	33 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1.75 min
	5′-CAGGGGTAAAACGCTCAACGATT