sopB |
5′-CGGGATCCGCGTTACGCAATCACTATC |
33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 5 min |
|
5′-GCGGCCGCCCGTTGACATCCTCCAGAA |
|
sopE |
5′-CGGGATCCTCTTGGCGCGTAGTCCTTC |
33 cycles of 94°C for 30 s, 52°C for 30 s, and 72°C for 4.5 min |
|
5′-TTTGCGGCCGCGCACTGGATACGCTGAACGA |
|
sopE2 |
5′-CGGGATCCGCGCAGGCGTTTAGAAGACAGTT |
33 cycles of 94°C for 30 s, 54°C for 30 s, and 72°C for 5 min |
|
5′-GCGGCCGCGTTCCAGCATCAGCCACTTG |
|
sipA |
5′-GCGGCCGCACCTGGGGTTGAGTCCTAC |
33 cycles of 94°C for 30 s, 58°C for 30 s, and 72°C for 6 min |
|
5′-CCCGGGACACCAAGGCACGAG |
|
invB |
5′-GGGCGCCAAGAGAAAAAGATGTC |
33 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1.75 min |
|
5′-CAGGGGTAAAACGCTCAACGATT |
|