FIG. 7.

Dual-channel images of 2D gels of extracellular proteins produced with the Delta2D software (Decodon GmbH), showing the differences in the protein patterns of the wild-type S. aureus strain COL (green) and the hemB mutant IA48 (red). The proteins in 100 μg of the supernatant from cells in the stationary growth phase were separated by preparative 2D polyacrylamide gel electrophoresis. The proteins were stained with silver nitrate. Proteins induced by a mutation in hemB are red, and proteins present only in the wild-type strain are green. The following proteins that were identified are indicated by arrows: glycerophosphoryl diester phosphodiesterase (GlpQ), α-hemolysin (Hla), β-hemolysin (Hlb), immunodominant antigen A (IsaA), leucocidine F (LukF), leucocidine M (LukM), thermonuclease (Nuc), 1-phosphatidylinositol phosphodiesterase (Plc), enterotoxin B (Seb), serinproteases (SplB and SplF), and a hypothetical protein (YfnI) (38).