. 2003 Dec;185(23):6883–6892. doi: 10.1128/JB.185.23.6883-6892.2003

TABLE 2.

Primers used in this study

Primer for PCR and sequencing	Sequence (5′-3′)	Position of the 5′ nucleotide
parE primers^a
GRLB1	CGCCGATAAGATAACTTAGTACTA	137
GRLB1R	GCTCTTGACGCTCTTTACCAC	1036
GRLB2	TAAGTGAACGACTACAAGAGTCTGC	947
GRLB2R	ACCTCCCGCAGAATCACCTTC	1692
GRLB3	GCAAGGGAAGCTGCACGTAAAGC	1537
GRLB3R	GGCAATGCACGCTCTTGAATA	2470
parC primers^a
GRLA1	AGTTGAAGATGAAGTGCGTT	2196
GRLA1R	ACCTTGAATAATACCACCAG	3041
GRLA2	GCTGAAGAGTTATTACGTGA	2757
GRLA2R	ACGCAAGATACCGTACTAGTA	4028
GRLA3	GTTATGGTGCCTAGTGAAGAA	3861
GRLA3R	TAACGATAAGTACTTGGTCAGC	4881
Primers for upstream region of gyrB^b
UPGB1	AGGTGACGACTCGGTAACGC	1,793 bp upstream of start site of gyrB
UPGB1R	AGGATATTCACGTCAGGATGACAT	1,039 bp upstream of start site of gyrB
UPGB2	TATCCCAGAATTACCTGAAGATG	1,178 bp upstream of start site of gyrB
UPGB2R	ATAGGATCGACTTCAGAGAGAG	127 bp downstream of start site of gyrB
gyrB primers^c
GYRB1	TAGACGATGTACTCAGTGAAT	2
GYRB1R	GTGGCATATCCTGAGTTATAT	1004
GYRB2	CTATCACTATGAGGGCGGTA	858
GYRB2R	GCACGAGCAACGATAACACTC	2252
gyrA primers
GYRA1	CCTCAATCAAGAATAAATGAACGA	2164
GYRA1R	TATCACGTAACAGTTCAAGTGT	2566
GYRA2	GGTTCAATGGATGGAGATGG	2482
GYRA2R	CTGTATCTGACTCACGAATC	3361
GYRA3	CGTACTGGTGTGCGTGTCGTT	3046
GYRA3R	CTGGCGTACGTTTACCATAAC	4336
GYRA4	CAACGGGTGTGAAAGGTAT	4211
GYRA4R	GCCACCATCAAGACTTATC	5027 (8)
norA promoter primers^d
DMBL3	CAATGCCGCTGACAGATGTA	27
DMBR3	TTGTCAGCTAGCGTACCACCAA	550
Primers for upstream region of topB^e
UPTB1	GTTATGGCTGATATAGGTGGTG	3,085 bp upstream of start site of topB
UPTB1R	CAAAACGCAATAACACATCATGAC	2,259 bp upstream of start site of topB
UPTB2	GGGCTTTTATTTATGACATAGTTG	2,446 bp upstream of start site of topB
UPTB2R	ACTTGTTCATGTTCTATTCTCA	1,922 bp upstream of start site of topB
UPTB3	GACGACGATCTTTTCTTTCTCC	2,047 bp upstream of start site of topB
UPTB3R	GGGGAGCACTTGCAAAAATA	1,215 bp upstream of start site of topB
UPTB4	CGTCGTACCACGAATT	1,368 bp upstream of start site of topB
UPTB4R	AGGCATTCAACAATACAAACCA	528 bp upstream of start site of topB
UPTB5	TGTAATTTGCTGAAAGCACGA	686 bp upstream of start site of topB
UPTB5R	CTAACGCCCACGTGACAATA	122 bp downstream of start site of topB
parE probe primers^a
GLBP	CAGGTTTTAGAGGGGTTAGAA	421
GLBPR	TGTCGGTTTACCTGATTTATG	654
parC probe primers^a
GRLAP	GATACGACACTCGAACCAAT	2817
GRLA1R	ACCTTGAATAATACCACCAG
gyrB probe primers^c
GYBP	CTATCACTATGAGGGCGGTATTA	858
GYBPR	GTATCTTCACCAGAAAGTCTATC	1124
gyrA probe primers
GYRA3	CGTACTGGTGTGCGTGTCGTT
GYRA2R	CTGTATCTGACTCACGAATC
topA probe primers^b
TOPAP	GCGTAATCCAGCAAACCCATT	747 bp from start site
TOPAPR	CACCTTGTTTCCCTGATGCTTT	1018 bp from start site
topB probe primers^b
TOPB3	AAGCCAATCCGTCGATTATG	370 bp from start site
TOPB3R	CGTTCCTGTCTGAACACGTC	582 bp from start site

Data were obtained from the sequence of Yamagishi et al. (52).

Data were obtained from the sequences of Baba et al. (4) and Kuroda et al. (32).

Data were obtained from the sequence of Ito et al. (25).

Data were obtained from the sequence of Yoshida et al. (53).

Data were obtained from the sequence of Baba et al. (4).