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Treatment with the prophage-inducing agent mitomycin C increases sak and sea transcript levels. Mitomycin C (300 ng/ml) was added to one of two identical MSSA476 cultures, and RNA was extracted from both cultures every 30 min for 2.5 h. Six micrograms of total RNA was loaded in each lane and subsequently probed with sak (a), sea (b), or dnaN (c). The dnaN blot acted as a control for RNA loading.
