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Creation of replication-deficient φSa3ms. (a) An internal fragment of ror was cloned into the temperature-sensitive shuttle vector pBT9, creating pPS79, and used to insertionally inactivate ror through plasmid integration into the MSSA476 chromosome. (b) Southern blot analysis comparing the amounts of φSa3ms DNA (sea probe) and chromosomal DNA (dnaN probe) in MSSA476 and MS79 (ror::pPS79) in the absence and presence of mitomycin C.
